Purification of Ascaris suum antigen: its allergenic activity in vitro and in vivo
LW Greenspon, J White, RL Shields, A Fügner… - Journal of allergy and …, 1986 - Elsevier
Crude aqueous extracts of Ascaris suum (CE) have been used widely to study IgE-mediated
reactions in various experimental preparations. Because some CE may contain a
polypeptide, a mast cell degranulating peptide (MCDP), that degranulates mast cells by
nonimmunologic mechanisms, 1, 2 various protocols have been used to ensure that the
Ascaris preparation used did not contain MCPD. In general, these protocols have assumed
MCDP had been removed without providing proof. Even protocols designed to isolate the …
reactions in various experimental preparations. Because some CE may contain a
polypeptide, a mast cell degranulating peptide (MCDP), that degranulates mast cells by
nonimmunologic mechanisms, 1, 2 various protocols have been used to ensure that the
Ascaris preparation used did not contain MCPD. In general, these protocols have assumed
MCDP had been removed without providing proof. Even protocols designed to isolate the …
