Brefeldin A, but not monensin, completely blocks CD69 expression on mouse lymphocytes:: efficacy of inhibitors of protein secretion in protocols for intracellular …
S Nylander, I Kalies - Journal of immunological methods, 1999 - Elsevier
Flow cytometry is increasingly used for cytokine detection where it serves to complement
ELISA (enzyme-linked immunosorbent assay) and ELISPOT assays. Since it is possible to
stain both extracellular epitopes and intracellular cytokines on the same cells, this is a
powerful technique for analysing cytokine expression in defined cell populations. However
unstimulated cells do not express cytokines. Thus, appropriate stimulation is a prerequisite
for studying cytokine expression. Here phorbol 12-myristate 13-acetate (PMA)/ionomycin in …
ELISA (enzyme-linked immunosorbent assay) and ELISPOT assays. Since it is possible to
stain both extracellular epitopes and intracellular cytokines on the same cells, this is a
powerful technique for analysing cytokine expression in defined cell populations. However
unstimulated cells do not express cytokines. Thus, appropriate stimulation is a prerequisite
for studying cytokine expression. Here phorbol 12-myristate 13-acetate (PMA)/ionomycin in …