[HTML][HTML] Membrane-specific, host-derived factors are required for US2-and US11-mediated degradation of major histocompatibility complex class I molecules
MH Furman, HL Ploegh, D Tortorella - Journal of Biological Chemistry, 2002 - ASBMB
Human cytomegalovirus encodes two glycoproteins, US2 and US11, that target major
histocompatibility complex (MHC) class I heavy chains for proteasomal degradation. We
have developed a mRNA-dependent cell-free system that recapitulates US2-and US11-
mediated degradation of MHC class I heavy chains. Microsomes support the degradation of
MHC class I heavy chains in the presence of US2 or US11 in a cytosol-dependent manner.
In vitro, the glycosylated heavy chain is exported from the microsomes. A deglycosylated …
histocompatibility complex (MHC) class I heavy chains for proteasomal degradation. We
have developed a mRNA-dependent cell-free system that recapitulates US2-and US11-
mediated degradation of MHC class I heavy chains. Microsomes support the degradation of
MHC class I heavy chains in the presence of US2 or US11 in a cytosol-dependent manner.
In vitro, the glycosylated heavy chain is exported from the microsomes. A deglycosylated …
