Cyclodextrin-catalyzed extraction of fluorescent sterols from monolayer membranes and small unilamellar vesicles
H Ohvo-Rekilä, B Åkerlund, JP Slotte - Chemistry and Physics of Lipids, 2000 - Elsevier
This study examined the kinetics of sterol desorption from monolayer and small unilamellar
vesicle membranes to 2-hydroxypropyl-β-cyclodextrin. The sterols used include cholesterol,
dehydroergosterol (ergosta-5, 7, 9,(11), 22-tetraen-3β-ol) and cholestatrienol (cholesta-5, 7,
9,(11)-trien-3β-ol). Desorption rates of dehydroergosterol and cholestatrienol from pure
sterol monolayers were faster (3.3–4.6-fold) than the rate measured for cholesterol. In mixed
monolayers (sterol: 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine 30: 70 mol%), both …
vesicle membranes to 2-hydroxypropyl-β-cyclodextrin. The sterols used include cholesterol,
dehydroergosterol (ergosta-5, 7, 9,(11), 22-tetraen-3β-ol) and cholestatrienol (cholesta-5, 7,
9,(11)-trien-3β-ol). Desorption rates of dehydroergosterol and cholestatrienol from pure
sterol monolayers were faster (3.3–4.6-fold) than the rate measured for cholesterol. In mixed
monolayers (sterol: 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine 30: 70 mol%), both …