Enzyme-cleaved antibodies are used widely for the treatment of envenoming. Such products
should comprise only 'highly pure'immunoglobulin fragments since Fc or other
contaminating protein fragments or their aggregates may lead to side effects. The digestion
of ovine antiserum and its purified IgG were investigated using pepsin and trypsin. Trypsin
was effective at digesting purified IgG but unsuitable for the direct digestion of serum. In
contrast, pepsin was highly effective at digesting all unwanted serum components to low …