Novel subtype of human angiotensin II type 1 receptor: cDNA cloning and expression
H Konishi, S Kuroda, Y Inada, Y Fujisawa - Biochemical and biophysical …, 1994 - Elsevier
H Konishi, S Kuroda, Y Inada, Y Fujisawa
Biochemical and biophysical research communications, 1994•ElsevierAngiotensin II (AII) plays a major role in regulation of cardiovascular function and fluid
homeostasis through the action of an AII type 1 receptor (AT 1 R). The cDNA encoding a
novel subtype of human AT 1 R (AT 1b R) was cloned from a human placental cDNA library.
The full-length cDNA clone (1563 bp) encoded a polypeptide that consists of 359 amino acid
(aa) residues with 97.2% aa identity to the human AT 1a R. All the aa replacements between
two human AT 1 Rs reside within the C-terminal half region of AT 1 R molecule. The 2.4-knt …
homeostasis through the action of an AII type 1 receptor (AT 1 R). The cDNA encoding a
novel subtype of human AT 1 R (AT 1b R) was cloned from a human placental cDNA library.
The full-length cDNA clone (1563 bp) encoded a polypeptide that consists of 359 amino acid
(aa) residues with 97.2% aa identity to the human AT 1a R. All the aa replacements between
two human AT 1 Rs reside within the C-terminal half region of AT 1 R molecule. The 2.4-knt …
Abstract
Angiotensin II (AII) plays a major role in regulation of cardiovascular function and fluid homeostasis through the action of an AII type 1 receptor (AT1R). The cDNA encoding a novel subtype of human AT1R (AT1bR) was cloned from a human placental cDNA library. The full-length cDNA clone (1563 bp) encoded a polypeptide that consists of 359 amino acid (aa) residues with 97.2% aa identity to the human AT1aR. All the aa replacements between two human AT1Rs reside within the C-terminal half region of AT1R molecule. The 2.4-knt AT1bR mRNA is expressed in the lung, placenta and Liver, and differs from AT1aR mRNA in its tissue distribution. The AT1bR expressed in COS-7 cells is pharmacologically distinct from the human AT1aR.
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