The Juxtaglomerular apparatus of the rat was studied after freeze-fracturing with special respect to intercellular junctions. It was found that Juxtaglomerular granulated cells of the vas afferens are interconnected by gap junctions to adjacent cells (granulated cells, possibly also smooth muscle cells). Gap junctions have also been found on the surface of lacis cells and mesangial cells. It is therefore concluded that these cells of the Juxtaglomerular apparatus and the glomerulus—granulated cells (possibly also smooth muscle cells) of the vas afferens, lacis cells and mesangium cells—form a functional system reacting in a coordinated manner to physiological stimuli.