Talin 2 is a large and complex gene encoding multiple transcripts and protein isoforms
E Debrand, Y El Jai, L Spence, N Bate… - The FEBS …, 2009 - Wiley Online Library
E Debrand, Y El Jai, L Spence, N Bate, U Praekelt, CA Pritchard, SJ Monkley, DR Critchley
The FEBS journal, 2009•Wiley Online LibraryTalins are large adaptor proteins that link the integrin family of adhesion molecules to F‐
actin. In vertebrates, there are two talin genes. Talin 1 is essential for integrin‐mediated cell
adhesion; the role of talin 2 is unclear. Here we report a detailed analysis of mammalian
talin 2. This reveals the existence of a previously unrecognized promoter associated with a
CpG island, and separated from the first coding exon by numerous alternatively spliced
noncoding exons spanning> 200 kb. Analysis of a mouse gene trap line shows that this …
actin. In vertebrates, there are two talin genes. Talin 1 is essential for integrin‐mediated cell
adhesion; the role of talin 2 is unclear. Here we report a detailed analysis of mammalian
talin 2. This reveals the existence of a previously unrecognized promoter associated with a
CpG island, and separated from the first coding exon by numerous alternatively spliced
noncoding exons spanning> 200 kb. Analysis of a mouse gene trap line shows that this …
Talins are large adaptor proteins that link the integrin family of adhesion molecules to F‐actin. In vertebrates, there are two talin genes. Talin 1 is essential for integrin‐mediated cell adhesion; the role of talin 2 is unclear. Here we report a detailed analysis of mammalian talin 2. This reveals the existence of a previously unrecognized promoter associated with a CpG island, and separated from the first coding exon by numerous alternatively spliced noncoding exons spanning > 200 kb. Analysis of a mouse gene trap line shows that this promoter accounts for most of the talin 2 expression in adult tissues. We also demonstrate that testis and kidney express truncated talin 2 isoforms that lack the N‐terminal half of the protein, and provide evidence for the developmentally regulated expression of the short testis‐specific talin 2 isoform in elongating spermatids. Finally, we identify four tissue‐specific alternative splicing events within the coding region of talin 2.
Wiley Online Library