The absorptive cells of the small intestine, enterocytes, are not generally thought of as a cell
type that stores triacylglycerols (TGs) in cytoplasmic lipid droplets (LDs). We revisit TG
metabolism in enterocytes by ex vivo and in vivo coherent anti-Stokes Raman scattering
(CARS) imaging of small intestine of mice during dietary fat absorption (DFA). We directly
visualized the presence of LDs in enterocytes. We determined lipid amount and quantified
LD number and size as a function of intestinal location and time post-lipid challenge via …