A facile stable-isotope dilution method for determination of sphingosine phosphate lyase activity

JH Suh, A Eltanawy, A Rangan, JD Saba - Chemistry and physics of lipids, 2016 - Elsevier
A new technique for quantifying sphingosine phosphate lyase activity in biological samples
is described. In this procedure, 2-hydrazinoquinoline is used to convert (2E)-hexadecenal
into the corresponding hydrazone derivative to improve ionization efficiency and selectivity
of detection. Combined utilization of liquid chromatographic separation and multiple reaction
monitoring-mass spectrometry allows for simultaneous quantification of the substrate S1P
and product (2E)-hexadecenal. Incorporation of (2E)-d 5-hexadecenal as an internal …