[PDF][PDF] Intrinsic requirement for zinc finger transcription factor Gfi-1 in neutrophil differentiation

H Hock, MJ Hamblen, HM Rooke, D Traver… - Immunity, 2003 - cell.com
H Hock, MJ Hamblen, HM Rooke, D Traver, RT Bronson, S Cameron, SH Orkin
Immunity, 2003cell.com
We report essential roles of zinc finger transcription factor Gfi-1 in myeloid development.
Gene-targeted Gfi-1−/− mice lack normal neutrophils and are highly susceptible to abscess
formation by gram-positive bacteria. Arrested, morphologically atypical, Gr1+ Mac1+ myeloid
cells expand with age in the bone marrow. RNAs encoding primary but not secondary or
tertiary neutrophil (granulocyte) granule proteins are expressed. The atypical Gr1+ Mac1+
cell population shares characteristics of both the neutrophil and macrophage lineages and …
Abstract
We report essential roles of zinc finger transcription factor Gfi-1 in myeloid development. Gene-targeted Gfi-1−/− mice lack normal neutrophils and are highly susceptible to abscess formation by gram-positive bacteria. Arrested, morphologically atypical, Gr1+Mac1+ myeloid cells expand with age in the bone marrow. RNAs encoding primary but not secondary or tertiary neutrophil (granulocyte) granule proteins are expressed. The atypical Gr1+Mac1+ cell population shares characteristics of both the neutrophil and macrophage lineages and exhibits phagocytosis and respiratory burst activity. Reexpression of Gfi-1 in sorted Gfi-1−/− progenitors ex vivo rescues neutrophil differentiation in response to G-CSF. Thus, Gfi-1 not only promotes differentiation of neutrophils but also antagonizes traits of the alternate monocyte/macrophage program.
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