[HTML][HTML] Protein kinase Cα is required for vanilloid receptor 1 activation: evidence for multiple signaling pathways
Z Olah, L Karai, MJ Iadarola - Journal of Biological Chemistry, 2002 - ASBMB
Activation of vanilloid receptor (VR1) by protein kinase C (PKC) was investigated in cells
ectopically expressing VR1 and primary cultures of dorsal root ganglion neurons.
Submicromolar phorbol 12, 13-dibutyrate (PDBu), which stimulates PKC, acutely activated
Ca 2+ uptake in VR1-expressing cells at pH 5.5, but not at mildly acidic or neutral pH. PDBu
was antagonized by bisindolylmaleimide, a PKC inhibitor, and ruthenium red, a VR1
ionophore blocker, but not capsazepine, a vanilloid antagonist indicating that catalytic …
ectopically expressing VR1 and primary cultures of dorsal root ganglion neurons.
Submicromolar phorbol 12, 13-dibutyrate (PDBu), which stimulates PKC, acutely activated
Ca 2+ uptake in VR1-expressing cells at pH 5.5, but not at mildly acidic or neutral pH. PDBu
was antagonized by bisindolylmaleimide, a PKC inhibitor, and ruthenium red, a VR1
ionophore blocker, but not capsazepine, a vanilloid antagonist indicating that catalytic …
