Transduction of Herpesvirus saimiri‐Transformed T Cells with Exogenous Genes of Interest
R Martínez‐Barricarte, SJ De Jong… - Current protocols in …, 2016 - Wiley Online Library
Current protocols in immunology, 2016•Wiley Online Library
Human T cells can be transformed and expanded with herpesvirus saimiri (HVS). HVS‐
transformed T cells from patients have facilitated the study of a broad range of primary
immunodeficiencies (PID) in which T‐cell development or function is altered. However, the
utility of HVS‐transformed T cells for genetic studies has been limited by technical
challenges in the expression of exogenous genes, including wild‐type or mutant alleles. A
novel, gamma retrovirus–based method for the simple and reliable transduction, purification …
transformed T cells from patients have facilitated the study of a broad range of primary
immunodeficiencies (PID) in which T‐cell development or function is altered. However, the
utility of HVS‐transformed T cells for genetic studies has been limited by technical
challenges in the expression of exogenous genes, including wild‐type or mutant alleles. A
novel, gamma retrovirus–based method for the simple and reliable transduction, purification …
Abstract
Human T cells can be transformed and expanded with herpesvirus saimiri (HVS). HVS‐transformed T cells from patients have facilitated the study of a broad range of primary immunodeficiencies (PID) in which T‐cell development or function is altered. However, the utility of HVS‐transformed T cells for genetic studies has been limited by technical challenges in the expression of exogenous genes, including wild‐type or mutant alleles. A novel, gamma retrovirus–based method for the simple and reliable transduction, purification, and study of HVS‐transformed T cells is described. © 2016 by John Wiley & Sons, Inc.
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