An improved method for collection of cerebrospinal fluid from anesthetized mice
NKH Lim, V Moestrup, X Zhang, WA Wang… - JoVE (Journal of …, 2018 - jove.com
The cerebrospinal fluid (CSF) is a valuable body fluid for analysis in neuroscience research.
It is one of the fluids in closest contact with the central nervous system and thus, can be used
to analyze the diseased state of the brain or spinal cord without directly accessing these
tissues. However, in mice it is difficult to obtain from the cisterna magna due to its closeness
to blood vessels, which often contaminate samples. The area for CSF collection in mice is
also difficult to dissect to and often only small samples are obtained (maximum of 5-7 µL or …
It is one of the fluids in closest contact with the central nervous system and thus, can be used
to analyze the diseased state of the brain or spinal cord without directly accessing these
tissues. However, in mice it is difficult to obtain from the cisterna magna due to its closeness
to blood vessels, which often contaminate samples. The area for CSF collection in mice is
also difficult to dissect to and often only small samples are obtained (maximum of 5-7 µL or …
