Protocol for rapid fluorescence in situ hybridization of bacteria in cryosections of microarthropods
T Thimm, CC Tebbe - Applied and environmental microbiology, 2003 - Am Soc Microbiol
T Thimm, CC Tebbe
Applied and environmental microbiology, 2003•Am Soc MicrobiolABSTRACT A protocol was developed to detect bacteria inhabiting microarthropods by
means of small-subunit rRNA-targeted fluorescence in situ hybridization and microscopy.
The protocol is based on cryosections of whole specimens. In contrast to more commonly
applied paraffin-embedding techniques, the protocol is quicker and reduces the number of
manipulations which might damage the microscopic material. The method allowed the study
of the bacterial colonization of Folsomia candida (Collembola) and the detection of bacteria …
means of small-subunit rRNA-targeted fluorescence in situ hybridization and microscopy.
The protocol is based on cryosections of whole specimens. In contrast to more commonly
applied paraffin-embedding techniques, the protocol is quicker and reduces the number of
manipulations which might damage the microscopic material. The method allowed the study
of the bacterial colonization of Folsomia candida (Collembola) and the detection of bacteria …
Abstract
A protocol was developed to detect bacteria inhabiting microarthropods by means of small-subunit rRNA-targeted fluorescence in situ hybridization and microscopy. The protocol is based on cryosections of whole specimens. In contrast to more commonly applied paraffin-embedding techniques, the protocol is quicker and reduces the number of manipulations which might damage the microscopic material. The method allowed the study of the bacterial colonization of Folsomia candida (Collembola) and the detection of bacteria in both the gut and tissue.
American Society for Microbiology