[HTML][HTML] Glycerol induces G6pc in primary mouse hepatocytes and is the preferred substrate for gluconeogenesis both in vitro and in vivo
KM Kalemba, Y Wang, H Xu, E Chiles… - Journal of Biological …, 2019 - ASBMB
Gluconeogenesis (GNG) is de novo production of glucose from endogenous carbon
sources. Although it is a commonly studied pathway, particularly in disease, there is a lack of
consensus about substrate preference. Moreover, primary hepatocytes are the current gold
standard for in vitro liver studies, but no direct comparison of substrate preference at
physiological fasting concentrations has been performed. We show that mouse primary
hepatocytes prefer glycerol to pyruvate/lactate in glucose production assays and 13 C …
sources. Although it is a commonly studied pathway, particularly in disease, there is a lack of
consensus about substrate preference. Moreover, primary hepatocytes are the current gold
standard for in vitro liver studies, but no direct comparison of substrate preference at
physiological fasting concentrations has been performed. We show that mouse primary
hepatocytes prefer glycerol to pyruvate/lactate in glucose production assays and 13 C …