[HTML][HTML] Hypoxic induction of prolyl 4-hydroxylase α (I) in cultured cells
Y Takahashi, S Takahashi, Y Shiga, T Yoshimi… - Journal of Biological …, 2000 - ASBMB
Accumulated evidence indicates that hypoxia activates collagen synthesis in tissues. To
explore the molecular mechanism of activation, we screened genes that are up-regulated or
down-regulated by hypoxia. Fibroblasts isolated from fetal rat lung were cultured under
hypoxia. Differential display technique showed that the mRNA level of prolyl 4-hydroxylase
(PH) α (I), an active subunit that catalyzes the oxygen-dependent hydroxylation of proline
residue in procollagen, increased 2–3-fold after an 8-h exposure to hypoxia. This elevated …
explore the molecular mechanism of activation, we screened genes that are up-regulated or
down-regulated by hypoxia. Fibroblasts isolated from fetal rat lung were cultured under
hypoxia. Differential display technique showed that the mRNA level of prolyl 4-hydroxylase
(PH) α (I), an active subunit that catalyzes the oxygen-dependent hydroxylation of proline
residue in procollagen, increased 2–3-fold after an 8-h exposure to hypoxia. This elevated …
