[HTML][HTML] Linkage-specific action of endogenous sialic acid O-acetyltransferase in Chinese hamster ovary cells
9-O-Acetylation of sialic acids shows cell type-specific and developmentally regulated
expression in various systems. In a given cell type, O-acetylation can also be specific to a
particular type of glycoconjugate. It is assumed that this regulation is achieved by control of
expression of specific 9-O-acetyltransferases. However, it has been difficult to test this
hypothesis, as these enzymes have so far proven intractable to purification or molecular
cloning. During a cloning attempt, we discovered that while polyoma T antigen-positive …
expression in various systems. In a given cell type, O-acetylation can also be specific to a
particular type of glycoconjugate. It is assumed that this regulation is achieved by control of
expression of specific 9-O-acetyltransferases. However, it has been difficult to test this
hypothesis, as these enzymes have so far proven intractable to purification or molecular
cloning. During a cloning attempt, we discovered that while polyoma T antigen-positive …