BA46 expression in different cell lines and patient tumor samples detected by RT-PCR. RNA from cell lines and fresh tumor samples was extracted, and cDNA was produced as described in Methods. The cDNA served as a template in PCR amplification with BA-46–specific primers. A 383-specific band was detected by gel electrophoresis. The lanes were as follows. (a) 1-kb marker, breast carcinomas: MDA-MB-157, MDA-MB-435, MCF7; ovarian carcinomas: SKOV, MLS, OC; bladder carcinomas: T24, sample 14, sample 19, RT4; colon carcinomas: sample 1, sample 2, sample 3, sample 4, HCT. (b) 1-kb marker, GPDH3, GPDH3, breast carcinoma: MDA-MB-231.